Intermediate

Part:BBa_I723029:Design

Designed by: Scott Ramsay   Group: iGEM07_Glasgow   (2007-10-26)


Pu plus RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 195
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Promoter cloned from the TOL plasmid from Pseudomonas putida MT2. This is the promoter that regulates the TOL catabolic plasmid upper pathway operon.

RBS taken from iGEM source plate.

References